Acute dermal toxicity study is used to identify potential side effects following skin application of a test substance. The test is performed after a single dose or multiple doses of the substance which have been applied to the skin over a 24-hour period.
Service details: In a typical design, two-five groups of female rats are dosed with 1 to 4 pre-selected doses of a test article. The test compounds are applied over the exposed area of dorsal/flank skin (~ 10 % of the total body surface area). Each compound-treated group as well as the vehicle dosing group contains 3-5 animals. Animals are observed for mortality and clinical signs of toxicity at 30 min, 2, 4, and 6 hours after the administration and thereafter daily for a period of 14 consecutive days. Individual bodу weights of animals are determined on the day of the compound administration and every two days thereafter. Gross necropsy is performed for all animals at the endpoint of the study. Specific tests, such as hematological, urine and blood clinical chemistry analysis, water and food intake, histopathology, etc. are available on request. Implementation of specialized endpoints based on the pharmacology of the test article is also available.