Recombinant proteins are an indispensable tool in drug discovery. The need for multiple forms of recombinant proteins, e.g. wild/mutant/fusion, from different species is increasing and we seek to fulfill the demand providing excellent service and support.
The production of recombinant proteins can be performed in:
- E.coli (multiple strains)
- mammalian cells (CHO, HEK293)
- Baculovirus-insect cells expression system
Depending on the aims of the study, we may assist in designing and producing expression constructs, or start the production of recombinant proteins with the ones provided by a customer.
To provide you with the best target for your experiments, the proteins can be tagged, biotinylated, or isolated as non-fused proteins. In addition, we offer:
- Activity measurements (biochemical or cell-based in vitro assays)
- Mass Spectrometry
- Purity assessment by SDS-PAAG, FPLC
- Thermal Shift Assay
- Evaluation of endotoxin contamination by the LAL assay
We thoroughly control the process of recombinant protein production at every step, starting from the cell transfection until measurement of a protein activity in vitro. We have all the required equipment to produce recombinant proteins of excellent quality, e.g. small-scale fermentation system, AKTA FPLC purification systems, temperature controlled incubator shakers, lyophylizers, etc. Bienta’s purified proteins exceed 90% purity as determined by SDS-PAGE, analytical chromatography and MS analysis, which would satisfy any requirements for research use.
For more details please contact us at firstname.lastname@example.org and we will be happy to answer any of your questions.