Bioanalytical Laboratory at Bienta offers a broad variety of in vitro ADME-Tox tests as well as pharmacokinetics studies in small animals.
The latest version of the Bienta Preclinical Services Catalog is available here .
Current ADME-Tox Capabilities
Physico-Chemical Properties
- Aqueous Solubility
- Shake flask solubility using Millipore or pION Solubility Filter Plates (UV or MS-MS detection)
- Laser nephelometry
- Chemical Stability Assay
- Log D/Log P
- Determining pKa
In vitro metabolism
- Metabolic stability in microsomes, hepatocytes and S9 from various sources (mouse, rat, human)
- CYP 450 inhibition assay (fluorogenic)
- CYP450 Time Dependent Inhibition (IC50 Shift Assay)
- LC-MS Based Cytochrome P450 Inhibition Assay
- Cytochrome CYP450 Reaction Phenotyping
- Plasma stability assay (human, rat, or mouse)
- Hepatic Microsomal Stability (human, rat, or mouse)
- Single Time Point Microsomal Stability Pre-Screen
- In Vitro Metabolite Profiling and Identification
In vitro permeability
- Membrane permeability
Protein binding studies
- Plasma Protein Binding Assay (Equilibrium Dialysis)
- Tissue Binding Assay (Equilibrium Dialysis)
- Microsomal Binding Assay (Equilibrium Dialysis)
In vitro toxicity studies
- Cytotoxicity
- various cell lines (HepG2 (hepatocarcinoma), SK-N-DZ (neuroblastoma), MCF-7 (breast adenocarcinoma) and others
- Bacterial reverse mutation test (Ames test)
- Micronucleus test
- FluxOR™ hERG Assay
Animal studies
We are constantly expanding the list of standard analytical services offered. In addition, our experienced bioanalytical group can develop custom services according to the client’s specifications. Entrusting ADMET/PK testing to Bienta is a natural choice for the chemistry clients of Enamine since it translates into the inherent time- and cost savings to the customer.
We also work with any compounds shipped in from the outside, full confidentiality guaranteed.