Subacute/subchronic (repeated-dose) toxicity (MTD/NOAEL)

Subacute systemic toxicity is defined as adverse effects occurring after multiple or continuous exposure between 24 h and 28 days. Subchronic systemic toxicity is defined as adverse effects occurring after the repeated or continuous administration of a test article for up to 90 days or not exceeding 10% of the animal’s lifespan.

Service details: In the standard design, one-three groups of animals of both sexes are dosed with 1-3 pre-selected doses of a test article. Each compound-treated group as well as vehicle dosing group contains 5 animals of each sex. Animals are observed daily for signs of toxicity, body weights are recorded weekly, and food intake can be measured weekly. At the end of the experimental period, blood samples are collected for hematology and clinical chemistry analysis, and a gross necropsy is performed at the endpoint of the study. Histopathology is also available upon request.

Treatment design and duration of the research depend on a therapeutic treatment schedule. The standard study design includes 1-3 tested doses per route in animals of both sexes, 5 animals per group, and Vehicle dosed group. On average, 40 animals and 28-90 working days are required for the study. A customized design is available.