High content imaging with confocal microscopy

Bienta is equipped with the fully automated confocal microscopy imaging system IN Cell Analyzer 6500HS from GE Healthcare 

Options for High Content Imaging confocal microscopy:

  • 384-well format as standard;  
  • 2-5 markers could be imaged simultaneously;  
  • adherent and suspension cell lines 
  • wide variety of cancer cell lines, and primary rat and mice cells are available at Bienta;  
  • IN Carta analysis software enables quick and robust quantitative analysis of images acquired on IN Cell Analyzer systems.

Examples of possible applications of IN Cell Analyzer 6500HS:  

  • Cell morphology changes (epithelial-to-mesenchymal transduction, cell differentiation);  
  • Organelles studies (number, size, localization, structure);  
  • Subcellular changes (translocation, trafficking, expression of proteins, co-localization of molecules);  
  • Cell viability;  
  • 3D cultures 
  • Co-cultures (cell-cell interactions including cancer-stroma); 
  • Cell migration and invasion (wound-healing assay, MMP activity);  
  • Imaging of tissue samples labeled with fluorescent dyes 

Figure 1.  3D cancer cell culture. Max intensity projection image of the HeLa tumoroid stained with Hoechst 33342.

Figure 2. Detection of the mitochondrial membrane potential in U-2 OS cells. Red – mitochondria stained with MitoTracker Red CMXRos, cyan – nuclei stained with Hoechst 33342.


Figure 3. Nuclear transcription factor translocation assay. Confocal image of HeLa cells stained with antibodies against transcription factor of interest (green), and Hoechst 33342 (blue).


Figure 4.  In vitro Micronucleus test. Confocal image of CHO-K1 cells treated with Mitomycin C. Cyan – nuclei and micronuclei of the cells were stained with Hoechst 33342, red – cytoplasm was labeled with Pyronin Y.


Figure 5. Apoptosis assay. Detection of the apoptosis using anti-cleaved caspase-3 antibodies (green) in HeLa cells treated with Staurosporine.