Tumor Necrosis Factor (TNF-α) is a proinflammatory cytokine with a significant ability to induce inflammation. Single parenteral administration of TNF-α is used as a model of sepsis and accompanying pathologies such as hypothermia, multiple organ injury including the lung, kidney, and other.
Service details: A standard study design includes at least 3 groups of male C57BL/6 mice (vehicle dosed, treated with reference compound, and tested compound treated group), 10 mice per group, 7 days of acclimatization period, a single injection of TNF-α, 3-4 days of the treatment period. Mice treatment procedures include intravenous TNF-α injection (50 μg endotoxin-free murine TNF-α alone or in combination with zVAD, body temperature was kept at 37°C to delay lethal shock), single or repeated dose drug administration. Mortality, body temperature, or customized clinical or physiological parameters are determined. Gross necropsy, specific tests, such as hematological, urinary, and blood clinical chemistry analysis, food intake, histopathology, etc. in combination with more definitive toxic or gross pathology endpoints are available on request.
Deliverable: A detailed study report including the description of the complete study design, full experimental data, and interpretation.