The animal models are essential for exploring acute renal failure (ARF) and the development of effective therapy for its optimal management. At Bienta we utilize several rodent models to mimic the clinical conditions of renal failure.

  • Cisplatin-induced acute kidney injury in mice 

    Cisplatin is widely used ...

    Cisplatin is a widely used anticancer drug accompanied by adverse side effects including toxin-induced acute kidney injury (AKI). Intraperitoneal administration of 10–12 mg/kg Cisplatin results in cellular uptake and efflux, apoptosis, vascular injury, oxidative and endoplasmic reticulum stress, and inflammation within 72 hours, followed by a slow recovery phase for 7 days. The severity of the pathology depends on the dose administered.Service details:
      A standard study design includes at least 3 groups of male C57BL/6 mice (vehicle dosed, treated with reference compound, and tested compound treated group), 10-15 mice per group, 7 days of acclimatization period, single Cisplatin injection, 3-10 days of the treatment period. Animals are monitored for mortality, bodyweight control changes, and signs of toxicity daily for the duration of treatment and post-treatment period.  Mice treatment procedures include invasive (by IV, IP, SC), and non-invasive (PO, and diet incorporated) routes of drug administration. Clinical chemistry parameters of renal failure (creatinine and urea in the blood) are determined over time (0, 2, 5, and 10 days after the administration of cisplatin). Gross necropsy is performed on all animals at the endpoint. Specific tests, such as hematological, urinary, and blood clinical chemistry analysis, food intake, histopathology, etc. in combination with specific endpoints are available on request.
  • Lipopolysaccharide (LPS) induced acute kidney injury in mice

    LPS is a potent inducer...

    LPS is a potent inducer of inflammation. Parenteral administration of LPS causes the production of a wide range of proinflammatory cytokines and is used as a model of sepsis. The inflammatory process also leads to hemodynamic changes and acute kidney injury. Intraperitoneal administration of 10 mg/kg LPS results in sepsis-associated acute renal damage within 24-72 hours. This is a rapid acute model, lasting 72-96 hours.Service details:
      A standard study design includes 3 groups of male C57BL/6 mice (vehicle dosed, treated with reference compound or intact healthy animals (optional), and tested compound treated group), 10-15 mice per group, 7 days of acclimatization period, single LPS injection, 1-3 days of the treatment period. Animals are observed for mortality and signs of toxicity (including body weight) at 30 min, 2, 4, and 6 hours after the first compound administration and thereafter daily for all treatment periods. Mice treatment procedures include invasive (by IV, IP, SC), and non-invasive (PO) routes of drug administration. Clinical chemistry parameters of renal failure (creatinine and urea in the blood) are determined. Gross necropsy is performed on all animals at the endpoint. Specific tests, such as hematological, urinary, and blood clinical chemistry analysis, food intake, histopathology, etc. in combination with more definitive toxic or gross pathology endpoints are available on request.
  • Gentamicin induced acute kidney injury in rats  

    Gentamicin is a broad-spectrum antibiotic...

    Gentamicin is a broad-spectrum antibiotic with side effects of toxin-induced acute kidney injury. Intraperitoneal administration of 100 mg/kg Gentamicin for four consecutive days results in renal tubular damage in rats, followed by a slow recovery phase for 14 days. The severity of the pathology depends on the dose administered.Service details:
      A standard study design includes at least 3 groups of male Sprague Dawley rats (vehicle dosed, treated with reference compound, and tested compound treated group), 8-10 rats per group, 7 days of acclimatization period, 4-7 days of Gentamicin treatment period, 4-14 days of treatment/post-treatment period. Animals are observed for mortality and signs of toxicity (including body weight) at 30 min, 2, 4, and 6 hours after the first compound administration and thereafter daily for the duration of treatment and post-treatment period. Rats treatment procedures include invasive (IV, IP, SC), or noninvasive (PO, and diet incorporated) routes of drug administration. Clinical chemistry parameters of renal failure (creatinine and urea in the blood) are determined over time (0, 2, 5, 10, 21 days after the first administration of Gentamicin). Gross necropsy is performed on all animals at the endpoint. Specific tests, such as hematological, urinary, and blood clinical chemistry analysis, food and water intake, histopathology, etc. in combination with more definitive toxic or gross pathology endpoints are available on request.
  • Renal ischemia-reperfusion injury model in rats  

    The Renal ischemia-reperfusion ...

    The Renal ischemia-reperfusion injury model (IRI) is achieved through applying ischemia on the kidney by clamping the renal artery and vein. Typically, renal vascular clamping for 50 minutes is used to induce renal injury, followed by reperfusion. The ischemia-reperfusion procedure leads to an acute decrease in renal function, uncovered by the level of urea and creatinine in the blood.Service details:
      A standard study design includes at least 3 groups of male Sprague Dawley rats (vehicle dosed, treated with reference compound, and tested compound treated group), 8-10 rats per group, 7 days of acclimatization period, one day IRI procedure, 1-7 days of treatment/post-treatment period. Animals are observed for mortality, body weight control, and signs of toxicity at 30 min, 2, 4, and 6 hours after the first compound administration and thereafter daily for all treatment and post-treatment periods. Rats treatment procedures include invasive (by IV, IP, SC), or noninvasive (PO, and diet incorporated) routes of drug administration. Clinical chemistry parameters of renal failure (creatinine and urea in the blood) are determined over time (0, 2, 5, 7 days after the IRI procedure). Gross necropsy is performed on all animals at the endpoint. Specific tests, such as hematological, urinary, and blood clinical chemistry analysis, food and water intake, histopathology, etc. in combination with more definitive toxic or gross pathology endpoints are available on request.
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