Spontaneously Hypertensive Rats (SHRs) is the best-investigated animal model of essential (or primary) hypertension used to study and treat cardiovascular disease. SH rats develop hypertension at the age of 5-6 weeks. Between 40 and 50 weeks, SHRs exhibit signs of vascular and cardiac hypertrophy, providing a classic model for studying the efficacy of new cardioprotective agents. 

The dynamics of diastolic blood pressure in SHR rats at different time-points after single PO administration of vehicle (20% Captisol) or Prazosine at a dose of 3 mg/kg (M±SD). Rats are placed in plastic holders set on the warming platform for 10 min to achieve stress-reducing and the tail temperature of 32-34°C before the testing. Systolic and diastolic blood pressure (BP) were measured. Mean BP and pulse pressure (PP, difference between systolic and diastolic BP) values were then calculated.

Service details: A standard study design includes at least 3 groups of rats (vehicle dosed, treated with reference compound, and tested compound treated group), 5-10 rats per group, 7 days of acclimatization period, 3-4 weeks of the treatment period, and 7 days of testing and post-treatment observation. Blood pressure is measured in the tail of a rat using volume pressure recording sensor technology with the CODA (Kent Scientific, USA) rat tail-cuff system. Rats treatment procedures include invasive (by IV, IP, SC routes), and non-invasive (PO route, and diet incorporated) routes of drug administration. Gross necropsy is performed on all animals at the endpoint. Specific tests, such as hematological, urinary, and blood clinical chemistry analysis, food and water intake, histopathology, etc. in combination with more definitive toxic or gross pathology endpoints are available on request.