Surface plasmon resonance (SPR) is a sensitive biophysical method for the secondary screening of low molecular weight compounds and hit confirmation. Advantages of SPR methods include a low amount of target protein consumption, real time and label free detection of the affinity and kinetics of molecular interactions.
Fig.1 Experimental setup of surface plasmon resonance assay
Shortly, SPR experiments involve capturing the target protein on the chip surface and detecting its interaction with compounds in the aqueous solution. To provide our customers with the best quality results, we prefer to use chips with neutravidin/streptavidin coated surface and biotinylated AviTag proteins. In this matter, we ensure uniform protein orientation and equivalent compound access to the active site of the protein. On request, the biotinylated protein can be produced at Bienta https://bienta.net/recombinant-protein-production.
We can provide study of binding kinetics and affinity analysis in a sensitive and highly efficient manner .
Fig.2 Principle of surface plasmon resonance assay
Our laboratory is equipped with state-of-the-art Biacore T200 instrument from GE Healthcare, which measures the small changes in the mass on the chip surface at the moment of interaction. Records about these changes are generated in sensorgrams that contain an information about association and dissociation of the compounds with the target protein.
SPR services for drug discovery:
- Experimental setup
- High-throughput screening:
- Single point 100 compounds/day
- Dose-response 10-30 compounds/day
- Data analysis