Introducing a new technology which is planned to be extensively used for screening projects at Bienta. Differential Scanning Fluorimetry (DSF, TSA) is a powerful biophysical method based on detecting the shifts in protein denaturation temperature upon ligand binding as reported by fluorescent dye interacting with protein core exposed by heat denaturation. DSF is a simple, label-free HTS technology applicable to most soluble proteins, irrespectively of their functions and activities. Now Bienta can provide on-site recombinant target protein production to support DSF-based screening projects.
absorption ADME assay biochemical bioequivalence channel chemoinformatics compound DELFIA development distribution docking Enamine excretion FLIPR FRET GPCR HTRF HTS in-silico ion kinase LANCE LC/MS library ligand membrane metabolism modeling molecular MS/MS permeability pharmacokinetics plate preclinical reader receptor screening software solubility targeted toxicity TR-FRET triple quadrupole virtual