The following murine cancer models are available at Bienta:

  • Syngeneic tumor mouse models are established with murine cancer cell lines in immuno-competent mice. Syngeneic mouse models are excellent for studying new anti-cancer therapeutic agents in the presence of a functional immune system. C3H, C57Bl/6J, or C57Bl/6N mice strains are used to establish the syngeneic model.
  • Xenograft tumor models are set up with human cancer cells inoculated in athymic (nude) or severe combined immune deficient (SCID) mice. The model can be delivered as a subcutaneous or orthotopic xenograft tumor. Xenograft models allow assessing the efficacy of a drug or therapy specifically against human cancer cells. CD1-Foxn1nu and SCID/beige are in-house, and other mouse strains are on-demand.Service details:  A standard study design includes 7 days of acclimatization period, 5-15 days of subcutaneous tumor growth, a study-specific treatment period, and post-treatment observation. Tumor size measurements are usually initiated on day 3-5 post-cancer cells inoculation and monitored daily using a caliper. A study design includes a minimum of 2 groups of mice (tumor-bearing vehicle-treated, and tumor-bearing compound-treated group), 10-15 mice per group. Drug administration can be performed either via invasive (by IV, IP, SC, and IM routes), or noninvasive (PO, IN, or with food/drink) routes. Other specific types of treatment are also possible, for example, photodynamic therapy (PDT) using a wide range of irradiation wavelengths.

Lewis Lung Carcinoma (LLC) growth dynamics in young male C57Bl/6j mice treated with anticancer compounds A, B, and C. 1×10*6 cells in 100 μl (50 μl DMEM+50 μl Matrigel)

Tumor treatment procedures include invasive (by IV, IP, SC, and PO routes), noninvasive (with food) routes of drug administration, as well photodynamic therapy (PDT) using a wide range of irradiation wavelengths.

LLC growth dynamics in young male C57Bl/6N mice treated with Photolon, and vehicle groups following the PDT. For all experimental groups irradiation was performed 1 hour after Photolon treatment at 14th day after tumor inoculation using the semiconductor laser “Lika-Khirurg M” (Fotonika Plus, Ukraine), and the following parameter settings: l=660 nm, P=250 mW/cm2, light dose = 225 J/cm2. Irradiation regime was controlled by Nova II Laser Power Meter (Ophir Optronics, Israel).

Orthotopicaly inoculated DU4475 growth dynamic in SCID/beige and CD1-Foxn1nu 6-7 weeks old females. 2×10*6 Cells in 100 μl (50 μl RPMI+50 μl Matrigel)

Deliverable: A detailed study report including description of study design, experimental data and interpretation, preliminary statistics, requested tissue samples and terminal blood.

Sample Submission:  Dry compound or compound in pre-made dosing formulation (amount required depends on the dosing levels and schedules). For example, for treating a group of 8 mice at 10 mg/kg, twice daily (b.i.d.) for 1 week, about 16 mg of the test compound is required.