Service details:

The following murine models of cancer are available at Bienta:

– syngeneic tumor growth models: strains C3H and C57Bl/6N;

– xenografts of human tumors in immunodeficient mice: nude, or SCID (severe combined immune deficient).

A standard study design includes 7 days of acclimatization period, 14 days of subcutaneous tumor growth, 7 days of treatment period, and 7 days of post-treatment observation. Tumor size measurements are initiated 9 days post inoculation and monitored three times per week using calipers. Study design includes 3 groups of mice (control, tumor-bearing vehicle treated, and tumor-bearing compound treated group), 10 mice per group.

Figure 2. Typical growth curve of B16F10 melanoma tumor in young male C57Bl/6N mice.

Tumor treatment procedures include invasive (by IV, IP, SC, and PO routes), noninvasive (with food) routes of drug administration, as well photodynamic therapy (PDT) using a wide range of irradiation wavelengths.

Figure 3. LLC growth dynamics in young male C57Bl/6N mice treated with Photolon, and vehicle groups following the PDT. For all experimental groups irradiation was performed 1 hour after Photolon treatment at 14th day after tumor inoculation using the semiconductor laser “Lika-Khirurg M” (Fotonika Plus, Ukraine), and the following parameter settings: l=660 nm, P=250 mW/cm2, light dose = 225 J/cm2. Irradiation regime was controlled by Nova II Laser Power Meter (Ophir Optronics, Israel).

Deliverable: A detailed study report including description of study design, experimental data and interpretation.

Sample Submission:  Dry compound or compound in pre-made dosing formulation (amount required depends on the dosing levels and schedules). For example, for treating a group of 8 mice at 10 mg/kg, twice daily (b.i.d.) for 1 week, about 16 mg of the test compound is required.