Project Manager (in vitro ADMET, pharmacokinetics and animal toxicity studies)

Due to a considerable expansion of our preclinical and in vivo services, we are seeking a talented self-motivated Project Manager to join our team. You will work in the area of Early Drug Discovery and will be managing projects on in vitro ADMET as well as pharmacokinetics and animal toxicity studies (in rodents).

Location: Kyiv, Ukraine

What We Offer:

  • Full-time employment in one of the top companies in the area;
  • Research and business opportunities in the area of preclinical drug development;
  • Career growth;
  • Competitive salary package.

Major Responsibilities include:

  • Coordinate complex research projects and collaboration between different work groups, including logistics, laboratory analysis, data processing and interpretation;
  • Effectively set up and meet project specific timelines and milestones;
  • Communicate with the Customers and provide expert support for projects;
  • Write and deliver reports for both commercial and internal projects.

Required Education and Experience:

  • Ph.D. degree in Biology/ Chemistry;
  • Experience in leading a research team and project management is an advantage.

Required Knowledge and Skills:

  • Understanding HPLC-MS and/or animal studies;
  • Ability to meet strict deadlines and effectively conduct simultaneous tasks;
  • Fluent English, both written and spoken;
  • Strong communication, organizational and project management skills;
  • Result-oriented approach in work;
  • Responsibility, persistence and accuracy.

To be considered for this job, please send your CV, cover letter and the anticipated salary, as well as at least two recommendation contacts, to the following address: petro.borysko@enamine.net.

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Formulation Screen

Background: Pharmacokinetics, in vivo pharmacology/efficacy and short-term toxicology are the typical animal studies crucial for prioritization of lead candidates in the early stages of drug discovery process. Selection of proper compound formulations meeting a variety of requirements, related to intrinsic physico-chemical properties of the compound, routes of delivery, stability, toxic side-effects, interference with bioanalytical methods etc., can have a substantial effect on the outcome of such studies. This issue is aggravated by the commonplace occurrence of poorly soluble NCEs in drug discovery.

Service Details: We will test solubilization of the test articles in 10-20 different formulation vehicles containing a variety of excipients commonly used in early preclinical formulations. Exact number of vehicles tested depends upon the properties of the compound and its behavior in the consecutive solubilization tests. Typically, 2 selected concentration levels can be tested through a dilution step. All formulations will be checked for full solubilization of the test article as well as the phase stability of each formulation over 24 hour period. This is accomplished by the visual observation using white-light transilluminator. Follow-up studies, such as longer-term chemical stability of the test article in the selected formulation, can be done for an additional fee.

Deliverable: Report containing compositions of the tested formulation vehicles, preparation of the formulations, solubilization and stability is provided.

Sample Submission: A minimal accurately weighted aliquot of dry compound (~1 mg) is required for each formulation recipe to be tested. We do not need to know the structures of the molecules for this test. However, some knowledge of the chemical nature of the tested compounds may facilitate the choice of most suitable formulation vehicles.

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New animal facility for in vivo studies launched at BIENTA

Bienta has just launched on-site laboratory for in vivo studies in rodents. This new animal facility allows us to continue expanding our services in preclinical toxicology and animal efficacy models, in addition to pharmacokinetics studies in mice and rats that Bienta was successfully offering for the last several years. Our immediate plans include setting up rodent efficacy models for the therapeutic areas of anti-infectives, inflammation and pain.

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Pharmacokinetic studies in mice or rats

Background and Service Details:

Pharmacokinetic  (PK)  studies  can  significantly  vary  in  design  depending  on  their  goals  and  parameters of  the  tested  compounds.  We offer PK in widely used mouse inbred (C57BL/6, BALB/c), or outbred (CD1, NMRI) strains, and Wistar, or Sprague Dawley rats.  All study protocols are reviewed by Bienta’s Institutional Animal Care and Use Committee (IACUC).

A typical PK study in mice involves two drug delivery routes (e.g. PO and IV), 6 time points for each route (for example: 5, 15, 30, 60, 120, 240 min for IV and 15, 30, 60, 120, 240 and 360 min for PO) and 4 animals per  each  time  point  group/route,  plus  the  common control  plasma  of Vehicle dosed group,  50  animals  in  total.  We will prepare blood plasma samples and measure compound concentrations by LC-MS/MS using AB Sciex API4000/3000 mass spectrometers and Shimadzu (Prominence, VP) HPLC systems.

We offer:

  • Measurements of various pharmacokinetic and pharmacodynamics parameters;

  • Compound absorption/eliminaton dynamics following different routes of administration;

  • Biodistribution (brain, liver, kidney, lung, spleen, heart, liquor, etc);

  • Metabolism/biotransformation – metabolite ID services;

  • Compound excretion dynamics (urine/feces, bile);

  • End-point or serial sampling;

  • Single compound or cassette pharmacokinetics for mix of 2-3-4-5 compounds;

  • Wide range of time points;

  • Native or perfused tissue sampling;

  • Development of  optimized  drug  delivery formulations to improve the bioavailability (Formulation Screen)

Deliverable: A detailed study report including full description of study design, analytical method, measured test article concentrations, all common PK parameters and PK graphs. Raw experimental data are available upon request.

Typical plasma concentration-time curve for single compound testing:

PK curve

Sample Submission.  Dry compound or compound in a pre-made animal dosing formulation. Amounts depend on the dosing levels. For a PK study in mice at 10 mg/kg PO and 10 mg/kg IV about 16 mg of compound is required. We do not need to know structures of the molecules for ADME testing. However, brutto formulas have to be provided for all studies involving MS detection.

Formulation Screen

 

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Bioanalytical Laboratory

Bioanalytical Laboratory is established to provide common in vitro ADMET (absorption, distribution, metabolism, excretion and toxicity) tests as well as pharmacokinetics studies in small animals either separately or as a component of integrated drug discovery projects including screening and/or medicinal chemistry services by Enamine… (more…)

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About Bienta

Bienta is a trademark for preclinical biology services of Enamine, the world’s largest supplier of drug-like molecules for drug discovery research. Bienta is operating as a Contract Research Organisation (CRO) providing biomolecular screening (HTS) services as well as comprehensive bioanalytical support of drug discovery projects, including in vitro ADMET (absorption, distribution, metabolism, excretion, toxicity) testing as well as pharmacokinetics (PK), toxicity and efficacy studies in animals.

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